Hotspot

AU144_gp052

IRb · 84859-85596 · 738 bp

Back to Echinochloa

Pi 0.00142

MarkerSeek score 49.20

Primer available yes

Label AU144_gp052

Feature Metadata

label_name: AU144_gp052
feature_type: gene
region: IRb
length_bp: 738
variable_sites: 3
pi: 0.00142
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
AU144_gp052_p1	GATTGAACAACCGGGATCCA	GTAAGGATTGCACCAGAGCA	50.0	50.0	57.9	57.9	915 bp	1.0	78.4
AU144_gp052_p2	GATTGAACAACCGGGATCCA	TAAGGATTGCACCAGAGCAC	50.0	50.0	57.9	57.9	914 bp	1.0	78.4
AU144_gp052_p3	GATTGAACAACCGGGATCCA	TCAACCCGAGTGCAATCTTT	50.0	45.0	57.9	57.7	943 bp	1.0	77.1
AU144_gp052_p4	GATTGAACAACCGGGATCCA	CGGTAAGGATTGCACCAGAG	50.0	55.0	57.9	58.3	917 bp	1.0	76.5
AU144_gp052_p5	GATTGAACAACCGGGATCCA	TCTGTCGGTAAGGATTGCAC	50.0	50.0	57.9	57.6	922 bp	1.0	76.2