Hotspot

ndhF-ycf1

SSC · 126820-126854 · 35 bp

Back to Echinacea

Pi 0.0379

MarkerSeek score 62.80

Primer available yes

Label ndhF-ycf1

Feature Metadata

label_name: ndhF-ycf1
feature_type: igs
region: SSC
length_bp: 35
variable_sites: 5
pi: 0.0379
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-ycf1_p1	GGGGGTGGACGTATTTCTTC	CTAGCACTGGGTAGACCTCA	55.0	55.0	58.0	57.9	434-478 bp	1.0	81.1
ndhF-ycf1_p2	GGGGGTGGACGTATTTCTTC	GCCGAGAAGAAGGTATCAGC	55.0	55.0	58.0	58.1	518-562 bp	1.0	80.7
ndhF-ycf1_p3	GGGGGTGGACGTATTTCTTC	TCGTTGTGGTCGGACTCTAT	55.0	50.0	58.0	58.2	622-666 bp	1.0	80.1
ndhF-ycf1_p4	GGGGGTGGACGTATTTCTTC	ATCAATTCGGTCGTTGTGGT	55.0	45.0	58.0	57.8	632-676 bp	1.0	80.0
ndhF-ycf1_p5	GGGGGTGGACGTATTTCTTC	AGAAGAAGGAGCCGAGAAGA	55.0	50.0	58.0	57.8	528-572 bp	1.0	80.0