Hotspot

trnL-UAG-rpl32

SSC · 122824-123579 · 756 bp

Back to Echinacea

Pi 0.00772
MarkerSeek score 59.60
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
756
variable_sites
15
pi
0.00772
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC AAAGGAAAGGATATGGGGCG 50.0 50.0 58.0 57.6 886-896 bp 1.0 78.8
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA AAAGGAAAGGATATGGGGCG 50.0 50.0 58.0 57.6 887-897 bp 1.0 78.8
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 868-878 bp 1.0 78.6
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 869-879 bp 1.0 78.6
trnL-UAG-rpl32_p5 TCCTAAGAGCAGCGTGTCTA AAAGGAAAGGATATGGGGCG 50.0 50.0 57.9 57.6 896-906 bp 1.0 77.9