Hotspot

rpl16-rps3

LSC · 81091-82272 · 1182 bp

Back to Echinacea

Pi 0.00122
MarkerSeek score 52.30
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
1182
variable_sites
5
pi
0.00122
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 CAAGTGCCTGAAGAGCGTAT GAGGGTAGGGTTCCACTACA 50.0 55.0 58.0 57.8 1407-1415 bp 1.0 78.7
rpl16-rps3_p2 CAAGTGCCTGAAGAGCGTAT AGAGGGTAGGGTTCCACTAC 50.0 55.0 58.0 57.5 1408-1416 bp 1.0 76.2
rpl16-rps3_p3 GGGTTCAAGTGCCTGAAGAG GAGGGTAGGGTTCCACTACA 55.0 55.0 58.5 57.8 1412-1420 bp 1.0 75.0
rpl16-rps3_p4 CAAGTGCCTGAAGAGCGTAT AGGGTAGGGTTCCACTACAA 50.0 50.0 58.0 57.3 1406-1414 bp 1.0 74.8
rpl16-rps3_p5 CAAGTGCCTGAAGAGCGTAT GAGAGGGTAGGGTTCCACTA 50.0 55.0 58.0 57.2 1409-1417 bp 1.0 73.9