Hotspot

petA-psbJ

LSC · 62532-63337 · 806 bp

Back to Echinacea

Pi 0.00496

MarkerSeek score 47.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 806
variable_sites: 9
pi: 0.00496
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.0	57.6	944-948 bp	1.0	77.5
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.9	57.6	934-938 bp	1.0	77.4
petA-psbJ_p3	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	45.0	50.0	58.0	57.4	982-986 bp	1.0	75.8
petA-psbJ_p4	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	45.0	50.0	58.0	57.4	981-985 bp	1.0	75.8
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	TGTGATCGGTTTAGTAGGCG	50.0	50.0	57.9	57.4	972-976 bp	1.0	75.7