Hotspot

ycf4-cemA

LSC · 59803-60652 · 850 bp

Back to Echinacea

Pi 0.00413

MarkerSeek score 44.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 850
variable_sites: 12
pi: 0.00413
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	1020-1030 bp	1.0	81.9
ycf4-cemA_p2	GAGCCATTCCATTGACTCGT	AAATCCACCAGGGCAAAAGT	50.0	45.0	58.0	57.9	1017-1027 bp	1.0	81.7
ycf4-cemA_p3	GAGCCATTCCATTGACTCGT	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.0	58.0	1371-1381 bp	1.0	81.5
ycf4-cemA_p4	GGGATCGCGGGTCTTTTTAT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	1261-1271 bp	1.0	81.4
ycf4-cemA_p5	GGGATCGCGGGTCTTTTTAT	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.0	58.0	1612-1622 bp	1.0	81.1