Hotspot

rpoC2-rps2

LSC · 23219-23476 · 258 bp

Back to Echinacea

Pi 0.00133

MarkerSeek score 49.70

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 258
variable_sites: 1
pi: 0.00133
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	TGCCTTTCAAAGAATTTCCATGA	CCATGGCCAAAATGAACTCC	34.8	50.0	57.2	57.3	363-374 bp	1.0	47.0
rpoC2-rps2_p2	TGCCTTTCAAAGAATTTCCATGA	GGCCAAAATGAACTCCTGCT	34.8	50.0	57.2	58.7	359-370 bp	1.0	46.5
rpoC2-rps2_p3	TCTTGCCTTTCAAAGAATTTCCA	CCATGGCCAAAATGAACTCC	34.8	50.0	57.1	57.3	366-377 bp	1.0	46.4
rpoC2-rps2_p4	TCTTGCCTTTCAAAGAATTTCCA	GGCCAAAATGAACTCCTGCT	34.8	50.0	57.1	58.7	362-373 bp	1.0	46.0
rpoC2-rps2_p5	TGCCTTTCAAAGAATTTCCATGA	TGGCCAAAATGAACTCCTGC	34.8	50.0	57.2	59.0	360-371 bp	1.0	44.2