Hotspot

psbI-trnS-GCU

LSC · 8307-8442 · 136 bp

Back to Echinacea

Pi 0.00899

MarkerSeek score 55.60

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 136
variable_sites: 4
pi: 0.00899
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GCAATCCTGGACGTGAAGAA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	58.2	58.1	249-258 bp	1.0	81.1
psbI-trnS-GCU_p2	CCAATGATCCAGGACGCAAT	ATTGGGAGAGATGGCTGAGT	50.0	50.0	58.3	58.1	264-273 bp	1.0	79.9
psbI-trnS-GCU_p3	GCAATCCTGGACGTGAAGAA	CGAGTTATTCGTACCGAGGG	50.0	55.0	58.2	57.6	197-206 bp	1.0	79.7
psbI-trnS-GCU_p4	CCAATGATCCAGGACGCAAT	CGAGTTATTCGTACCGAGGG	50.0	55.0	58.3	57.6	212-221 bp	1.0	78.4
psbI-trnS-GCU_p5	GCAATCCTGGACGTGAAGAA	GATGGCTGAGTGGACGAAAG	50.0	55.0	58.2	58.6	240-249 bp	1.0	77.0