Hotspot

trnH-GUG-psbA

LSC · 80-457 · 378 bp

Back to Echinacea

Pi 0.00764

MarkerSeek score 48.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 378
variable_sites: 10
pi: 0.00764
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	55.0	55.0	58.1	58.3	490-502 bp	1.0	79.3
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	504-516 bp	1.0	79.2
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	503-515 bp	1.0	79.2
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	505-517 bp	1.0	79.2
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	55.0	50.0	58.1	57.6	491-503 bp	1.0	78.8