Hotspot

ycf1

SSC · 122274-127751 · 5478 bp

Back to Echinacanthus

Pi 0.056

MarkerSeek score 72.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5478
variable_sites: 454
pi: 0.056
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGTCTATATTTATGAGATAAGACTC	TGAAAGAAACCTCAAAAACGGA	30.8	36.4	53.9	56.7	172-185 bp	1.0	48.5
ycf1_p2	TCGTCTATATTTATGAGATAAGACTC	ACTGAAAGAAACCTCAAAAACGG	30.8	39.1	53.9	58.0	174-187 bp	1.0	48.4
ycf1_p3	TCGTCTATATTTATGAGATAAGACT	ACTGAAAGAAACCTCAAAAACGG	28.0	39.1	52.8	58.0	174-187 bp	1.0	48.4
ycf1_p4	TCGTCTATATTTATGAGATAAGACTC	CTGAAAGAAACCTCAAAAACGGA	30.8	39.1	53.9	57.8	173-186 bp	1.0	48.4
ycf1_p5	CGTCTATATTTATGAGATAAGACTC	ACTGAAAGAAACCTCAAAAACGG	32.0	39.1	52.6	58.0	173-186 bp	1.0	48.4