Hotspot

rrn16

IRa · 134870-136360 · 1491 bp

Back to Echinacanthus

Pi 0.102
MarkerSeek score 86.60
Primer available yes
Label rrn16

Feature Metadata

label_name
rrn16
feature_type
rRNA
region
IRa
length_bp
1491
variable_sites
229
pi
0.102
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
86.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16_p1 CGAATCCGCTTTGTCTACGA TGTGAAGCAGTGTCAAACCA 50.0 45.0 58.1 57.9 1592-1656 bp 1.0 88.7
rrn16_p2 CGAATCCGCTTTGTCTACGA TGTTTTGGGTGTGAAGCAGT 50.0 45.0 58.1 58.2 1601-1665 bp 1.0 88.5
rrn16_p3 CGAATCCGCTTTGTCTACGA TTTTGGGTGTGAAGCAGTGT 50.0 45.0 58.1 58.2 1599-1663 bp 1.0 88.5
rrn16_p4 CGAATCCGCTTTGTCTACGA GTTTTGGGTGTGAAGCAGTG 50.0 50.0 58.1 57.8 1600-1664 bp 1.0 88.0
rrn16_p5 CGCTTTGTCTACGAACAAGC TGTGAAGCAGTGTCAAACCA 50.0 45.0 57.8 57.9 1586-1650 bp 1.0 87.8
rrn16_p1 TGTGAAGCAGTGTCAAACCA CGAATCCGCTTTGTCTACGA 45.0 50.0 57.9 58.1 1592-1656 bp 1.0 88.5
rrn16_p2 TGTTTTGGGTGTGAAGCAGT CGAATCCGCTTTGTCTACGA 45.0 50.0 58.2 58.1 1601-1665 bp 1.0 88.3
rrn16_p3 TTTTGGGTGTGAAGCAGTGT CGAATCCGCTTTGTCTACGA 45.0 50.0 58.2 58.1 1599-1663 bp 1.0 88.3
rrn16_p4 GTTTTGGGTGTGAAGCAGTG CGAATCCGCTTTGTCTACGA 50.0 50.0 57.8 58.1 1600-1664 bp 1.0 87.8
rrn16_p5 TGTGAAGCAGTGTCAAACCA CGCTTTGTCTACGAACAAGC 45.0 50.0 57.9 57.8 1586-1650 bp 1.0 87.6