Hotspot

ndhC-trnV(UAC)

LSC · 50573-51622 · 1050 bp

Back to Echinacanthus

Pi 0.0512

MarkerSeek score 83.60

Primer available yes

Label ndhC-trnV(UAC)

Feature Metadata

label_name: ndhC-trnV(UAC)
feature_type: igs
region: LSC
length_bp: 1050
variable_sites: 78
pi: 0.0512
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV(UAC)_p1	TCATATTCGTAAAGCAAAAACA	GTTCGAGTCCGTATAGCCCT	27.3	55.0	52.7	58.7	1094-1110 bp	1.0	49.0
ndhC-trnV(UAC)_p2	TCATATTCGTAAAGCAAAAACA	AGAAGGTCTACGGTTCGAGT	27.3	50.0	52.7	57.8	1106-1122 bp	1.0	48.9
ndhC-trnV(UAC)_p3	TCATATTCGTAAAGCAAAAACA	GAAGGTCTACGGTTCGAGTC	27.3	55.0	52.7	57.5	1105-1121 bp	1.0	48.9
ndhC-trnV(UAC)_p4	ATCATATTCGTAAAGCAAAAACA	AGAAGGTCTACGGTTCGAGT	26.1	50.0	53.3	57.8	1107-1123 bp	1.0	48.9
ndhC-trnV(UAC)_p5	TCATATTCGTAAAGCAAAAACAT	AGAAGGTCTACGGTTCGAGT	26.1	50.0	53.3	57.8	1106-1122 bp	1.0	48.9