Hotspot

trnG(GCC)-trnfM(CAU)

LSC · 36631-36798 · 168 bp

Back to Echinacanthus

Pi 0.0646

MarkerSeek score 82.00

Primer available yes

Label trnG(GCC)-trnfM(CAU)

Feature Metadata

label_name: trnG(GCC)-trnfM(CAU)
feature_type: igs
region: LSC
length_bp: 168
variable_sites: 15
pi: 0.0646
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG(GCC)-trnfM(CAU)_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	231-278 bp	1.0	88.1
trnG(GCC)-trnfM(CAU)_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	232-279 bp	1.0	88.1
trnG(GCC)-trnfM(CAU)_p3	AGAAGATGCGGGTTCGATTC	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.1	58.1	225-272 bp	1.0	87.6
trnG(GCC)-trnfM(CAU)_p4	AGAAGATGCGGGTTCGATTC	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	267-314 bp	1.0	87.2
trnG(GCC)-trnfM(CAU)_p5	GAGAAGATGCGGGTTCGATT	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	268-315 bp	1.0	87.2