Hotspot

trnE(UUC)-trnT(GGU)

LSC · 31007-31621 · 615 bp

Back to Echinacanthus

Pi 0.0509

MarkerSeek score 82.40

Primer available yes

Label trnE(UUC)-trnT(GGU)

Feature Metadata

label_name: trnE(UUC)-trnT(GGU)
feature_type: igs
region: LSC
length_bp: 615
variable_sites: 46
pi: 0.0509
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE(UUC)-trnT(GGU)_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	719-741 bp	1.0	85.0
trnE(UUC)-trnT(GGU)_p2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	698-720 bp	1.0	84.8
trnE(UUC)-trnT(GGU)_p3	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.8	58.1	695-717 bp	1.0	84.6
trnE(UUC)-trnT(GGU)_p4	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	716-738 bp	1.0	83.9
trnE(UUC)-trnT(GGU)_p5	CGTTGCCTCCTTGAAAGAGA	TTGAACCGATGACTTACGCC	50.0	50.0	57.8	58.3	721-743 bp	1.0	82.8