Hotspot

trnD(GUC)-trnY(GUA)

LSC · 30711-30790 · 80 bp

Back to Echinacanthus

Pi 0.0684

MarkerSeek score 81.70

Primer available yes

Label trnD(GUC)-trnY(GUA)

Feature Metadata

label_name: trnD(GUC)-trnY(GUA)
feature_type: igs
region: LSC
length_bp: 80
variable_sites: 8
pi: 0.0684
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD(GUC)-trnY(GUA)_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	150-158 bp	1.0	79.4
trnD(GUC)-trnY(GUA)_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	149-157 bp	1.0	79.4
trnD(GUC)-trnY(GUA)_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	171-179 bp	1.0	77.9
trnD(GUC)-trnY(GUA)_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	170-178 bp	1.0	77.7
trnD(GUC)-trnY(GUA)_p5	CGGTGCTCTGACCAATTGAA	CGGACTGTAAATTCGTTGGC	50.0	50.0	58.5	57.5	166-174 bp	1.0	76.7