Hotspot

trnC(GCA)-petN

LSC · 28053-28941 · 889 bp

Back to Echinacanthus

Pi 0.0575

MarkerSeek score 81.80

Primer available yes

Label trnC(GCA)-petN

Feature Metadata

label_name: trnC(GCA)-petN
feature_type: igs
region: LSC
length_bp: 889
variable_sites: 71
pi: 0.0575
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC(GCA)-petN_p1	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCGAGATTTACT	50.0	50.0	58.1	58.0	946-959 bp	1.0	89.1
trnC(GCA)-petN_p2	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	999-1012 bp	1.0	88.9
trnC(GCA)-petN_p3	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	997-1010 bp	1.0	88.9
trnC(GCA)-petN_p4	TTTCCCCAGTTCAAATCCGG	AGGTCCGGTTTTCCTCACTA	50.0	50.0	58.1	58.0	1329-1352 bp	0.666667	73.9
trnC(GCA)-petN_p5	TTTCCCCAGTTCAAATCCGG	AAAGGTCCGGTTTTCCTCAC	50.0	50.0	58.1	58.0	1331-1354 bp	0.666667	73.9