Hotspot

rps16-trnQ(UUG)

LSC · 5753-6848 · 1096 bp

Back to Echinacanthus

Pi 0.0612
MarkerSeek score 84.20
Primer available yes
Label rps16-trnQ(UUG)

Feature Metadata

label_name
rps16-trnQ(UUG)
feature_type
igs
region
LSC
length_bp
1096
variable_sites
94
pi
0.0612
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.94
markerseek_score
84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ(UUG)_p1 TGCTTTCTACCACATCGTTTCA GAGGTTCGAATCCTTCCGTC 40.9 55.0 58.3 58.1 1129-1172 bp 1.0 71.8
rps16-trnQ(UUG)_p2 GCTTTCTACCACATCGTTTCA GAGGTTCGAATCCTTCCGTC 42.9 55.0 56.6 58.1 1128-1171 bp 1.0 70.4
rps16-trnQ(UUG)_p3 TGCTTTCTACCACATCGTTTC GAGGTTCGAATCCTTCCGTC 42.9 55.0 56.6 58.1 1129-1172 bp 1.0 70.4
rps16-trnQ(UUG)_p4 TGCTTTCTACCACATCGTTT GAGGTTCGAATCCTTCCGTC 40.0 55.0 55.3 58.1 1129-1172 bp 1.0 68.3
rps16-trnQ(UUG)_p5 GCTTTCTACCACATCGTTTCAA GAGGTTCGAATCCTTCCGTC 40.9 55.0 57.3 58.1 1128-1171 bp 1.0 68.0