Hotspot

ccsA

SSC · 118683-119642 · 960 bp

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Pi 0.00437

MarkerSeek score 42.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 960
variable_sites: 12
pi: 0.00437
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCGGGACCACGAGAATTATT	CTTGAGCATCTCGGTTCGAA	50.0	50.0	58.1	58.0	1446-1451 bp	1.0	81.6
ccsA_p2	GGGCATGAGTGGTTTTGTTG	CTTGAGCATCTCGGTTCGAA	50.0	50.0	57.8	58.0	1648-1653 bp	1.0	80.7
ccsA_p3	GCTCTTGACGACTGCAACTA	CTTGAGCATCTCGGTTCGAA	50.0	50.0	57.9	58.0	1147-1148 bp	1.0	80.6
ccsA_p4	GCGGGACCACGAGAATTATT	TAGACACGCTGCTCTTAGGA	50.0	50.0	58.1	57.9	1473-1478 bp	1.0	80.6
ccsA_p5	GCTCTTGACGACTGCAACTA	TAGACACGCTGCTCTTAGGA	50.0	50.0	57.9	57.9	1174-1175 bp	1.0	79.6