Hotspot

ndhD-ccsA

SSC · 118433-118682 · 250 bp

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Pi 0.0305
MarkerSeek score 66.10
Primer available yes
Label ndhD-ccsA

Feature Metadata

label_name
ndhD-ccsA
feature_type
igs
region
SSC
length_bp
250
variable_sites
17
pi
0.0305
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-ccsA_p1 TGGTATTTACCCGGATTTCGT AGGGCTACATAGTTATGGTTCA 42.9 40.9 57.1 56.3 367-372 bp 1.0 45.0
ndhD-ccsA_p2 TGGTATTTACCCGGATTTCGTT AGGGCTACATAGTTATGGTTCA 40.9 40.9 57.8 56.3 367-372 bp 1.0 45.0
ndhD-ccsA_p3 TGGTATTTACCCGGATTTCGT AGGGCTACATAGTTATGGTTCAT 42.9 39.1 57.1 56.8 367-372 bp 1.0 45.0
ndhD-ccsA_p4 TGGTATTTACCCGGATTTCGT GGGCTACATAGTTATGGTTCA 42.9 42.9 57.1 54.8 366-371 bp 1.0 45.0
ndhD-ccsA_p5 TTGGTATTTACCCGGATTTCGT AGGGCTACATAGTTATGGTTCA 40.9 40.9 57.8 56.3 368-373 bp 1.0 44.9