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psbE-petL

LSC · 62465-63538 · 1074 bp

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Pi 0.00975

MarkerSeek score 58.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1074
variable_sites: 32
pi: 0.00975
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGTAAAAACCCGAAATAACCTGT	50.0	34.8	57.9	56.4	1196-1253 bp	1.0	46.7
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGTAAAAACCCGAAATAACCTGT	50.0	34.8	57.8	56.4	1119-1176 bp	1.0	46.2
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AAAAACCCGAAATAACCTGT	50.0	35.0	57.9	53.3	1193-1250 bp	1.0	45.9
psbE-petL_p4	CAACCCGCAATGAATAGGGA	AAAACCCGAAATAACCTGTT	50.0	35.0	57.9	53.3	1192-1249 bp	1.0	45.9
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGTAAAAACCCGAAATAACCTGT	45.0	34.8	58.2	56.4	1205-1262 bp	1.0	45.8