Hotspot

trnF-GAA-ndhJ

LSC · 46888-47534 · 647 bp

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Pi 0.0177

MarkerSeek score 65.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 647
variable_sites: 35
pi: 0.0177
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCCCGCTTGAAACGTATC	55.0	50.0	58.0	58.1	820-830 bp	1.0	83.5
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCCCGCTTGAAACGTATC	50.0	50.0	58.1	58.1	784-794 bp	1.0	83.2
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATCCCCGCTTGAAACGTATC	55.0	50.0	57.9	58.1	808-818 bp	1.0	82.5
trnF-GAA-ndhJ_p4	GAAAGGATGGTCGGGATAGC	ATCCCCGCTTGAAACGTATC	55.0	50.0	57.8	58.1	832-842 bp	1.0	82.1
trnF-GAA-ndhJ_p5	AAAGGATGGTCGGGATAGCT	ATCCCCGCTTGAAACGTATC	50.0	50.0	58.2	58.1	831-841 bp	1.0	81.9