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psaA-ycf3

LSC · 41370-42015 · 646 bp

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Pi 0.00568

MarkerSeek score 63.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 646
variable_sites: 10
pi: 0.00568
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	CAGAGGCTTGGTTCGATCAA	50.0	50.0	57.9	57.9	839-869 bp	1.0	80.5
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	753-783 bp	1.0	80.2
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	ATAGCACTTACTCCCGGGAA	50.0	50.0	57.9	57.8	795-825 bp	1.0	80.1
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TAGCACTTACTCCCGGGAAT	50.0	50.0	57.9	57.8	794-824 bp	1.0	80.1
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	757-787 bp	1.0	80.0