Hotspot

rpoB-trnC-GCA

LSC · 26833-27987 · 1155 bp

Back to Echeveria

Pi 0.00979

MarkerSeek score 59.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1155
variable_sites: 33
pi: 0.00979
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TTTCCCTCATTTCCATCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.6	58.1	1215-1243 bp	1.0	79.9
rpoB-trnC-GCA_p2	TTTCCCTCATTTCCATCCCG	GTCTTGTGTTGATCAGGCGA	50.0	50.0	57.6	58.2	1238-1266 bp	1.0	78.8
rpoB-trnC-GCA_p3	TTTCCCTCATTTCCATCCCG	TCTTGTGTTGATCAGGCGAC	50.0	50.0	57.6	58.2	1237-1265 bp	1.0	78.8
rpoB-trnC-GCA_p4	TTTCCCTCATTTCCATCCCG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.6	58.4	1197-1225 bp	1.0	77.8
rpoB-trnC-GCA_p5	TTTCCCTCATTTCCATCCCG	CTTGTGTTGATCAGGCGACA	50.0	50.0	57.6	58.5	1236-1264 bp	1.0	76.6