Hotspot

trnS-GCU-trnG-GCC

LSC · 8551-9194 · 644 bp

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Pi 0.0154

MarkerSeek score 66.10

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 644
variable_sites: 28
pi: 0.0154
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	TGTTCGGAATACAAAGAAAACCG	50.0	39.1	58.0	57.9	755-768 bp	1.0	60.2
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	TGTTCGGAATACAAAGAAAACCG	50.0	39.1	58.0	57.9	754-767 bp	1.0	60.2
trnS-GCU-trnG-GCC_p3	GCTCGTACAACGGATTAGCA	TGTTCGGAATACAAAGAAAACCG	50.0	39.1	58.1	57.9	773-786 bp	1.0	59.5
trnS-GCU-trnG-GCC_p4	CAATCCGACGCTTTAGTCCA	CGAAGGACCCCTTAACTATTAGG	50.0	47.8	58.0	57.8	734-747 bp	1.0	59.5
trnS-GCU-trnG-GCC_p5	AATCCGACGCTTTAGTCCAC	CGAAGGACCCCTTAACTATTAGG	50.0	47.8	58.0	57.8	733-746 bp	1.0	59.5