Hotspot

psbK-psbI

LSC · 7807-8192 · 386 bp

Back to Echeveria

Pi 0.0137

MarkerSeek score 60.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 16
pi: 0.0137
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	CTTCTCGTCCAGGATTACGTC	50.0	52.4	57.9	57.9	521-522 bp	1.0	73.6
psbK-psbI_p2	CCAAATTACCCGAGGCCTAC	CTTCTCGTCCAGGATTACGTC	55.0	52.4	58.0	57.9	607-608 bp	1.0	73.4
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TTCTCGTCCAGGATTACGTC	50.0	50.0	57.9	56.8	520-521 bp	1.0	72.4
psbK-psbI_p4	CCAAATTACCCGAGGCCTAC	TTCTCGTCCAGGATTACGTC	55.0	50.0	58.0	56.8	606-607 bp	1.0	72.2
psbK-psbI_p5	CCAAATTACCCGAGGCCTAC	CTTCTCGTCCAGGATTACGT	55.0	50.0	58.0	56.8	607-608 bp	1.0	72.1