Hotspot

psbN

Genome · 14645-14776 · 132 bp

Back to Dystaenia

Pi 0.214

MarkerSeek score 66.70

Primer available yes

Label psbN

Feature Metadata

label_name: psbN
feature_type: gene
region: Genome
length_bp: 132
variable_sites: 3
pi: 0.214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.11
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN_p1	CATTGAGAGCTGTTGACTTTGT	GAGAACCGCCTAAAGTTCCA	40.9	50.0	57.2	57.5	337 bp	0.5	33.7
psbN_p2	TTGAGAGCTGTTGACTTTGT	GAGAACCGCCTAAAGTTCCA	40.0	50.0	55.1	57.5	335 bp	0.5	33.3
psbN_p3	CATTGAGAGCTGTTGACTTTGT	AACCGCCTAAAGTTCCAACT	40.9	45.0	57.2	57.3	334 bp	0.5	32.2
psbN_p4	TTGAGAGCTGTTGACTTTGT	AACCGCCTAAAGTTCCAACT	40.0	45.0	55.1	57.3	332 bp	0.5	31.9
psbN_p5	TCATTGAGAGCTGTTGACTTTG	GAGAACCGCCTAAAGTTCCA	40.9	50.0	56.9	57.5	338 bp	0.5	31.6