Hotspot

cemA

Genome · 80788-81477 · 690 bp

Back to Dystaenia

Pi 0.00435

MarkerSeek score 61.20

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: Genome
length_bp: 690
variable_sites: 3
pi: 0.00435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	TTGGATGGCGTAGAGTCAAC	ACATGCTTTGCTTCTGTACAAC	50.0	40.9	57.6	57.8	828 bp	1.0	59.4
cemA_p2	TGGATGGCGTAGAGTCAACT	ACATGCTTTGCTTCTGTACAAC	50.0	40.9	58.4	57.8	827 bp	1.0	58.9
cemA_p3	TTGGATGGCGTAGAGTCAAC	ACATGCTTTGCTTCTGTACA	50.0	40.0	57.6	55.6	828 bp	1.0	57.9
cemA_p4	TGGATGGCGTAGAGTCAACT	ACATGCTTTGCTTCTGTACA	50.0	40.0	58.4	55.6	827 bp	1.0	57.4
cemA_p5	TTGGATGGCGTAGAGTCAAC	TGCTTTGCTTCTGTACAACT	50.0	40.0	57.6	55.5	825 bp	1.0	57.3