Hotspot

rpl32-trnL-UAG

Genome · 117290-118227 · 938 bp

Back to Dystaenia

Pi 0.00975
MarkerSeek score 58.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
Genome
length_bp
938
variable_sites
9
pi
0.00975
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGGATATTGGGCAGCCTTA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 1057-1062 bp 1.0 80.4
rpl32-trnL-UAG_p2 AGGGATATTGGGCAGCCTTA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 1058-1063 bp 1.0 80.4
rpl32-trnL-UAG_p3 AGGGATATTGGGCAGCCTTA CTCGCACTGCTTCCTAAGAG 50.0 55.0 58.2 58.1 1078-1083 bp 1.0 79.8
rpl32-trnL-UAG_p4 AGGGATATTGGGCAGCCTTA CTCTCGCACTGCTTCCTAAG 50.0 55.0 58.2 58.1 1080-1085 bp 1.0 79.8
rpl32-trnL-UAG_p5 AGGGATATTGGGCAGCCTTA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 1067-1072 bp 1.0 79.5