Hotspot

psaA-ycf3

Genome · 61826-62521 · 696 bp

Back to Dystaenia

Pi 0.00432

MarkerSeek score 61.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: Genome
length_bp: 696
variable_sites: 3
pi: 0.00432
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	816-826 bp	1.0	78.6
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	803-813 bp	1.0	78.2
psaA-ycf3_p3	TTCTTGAGAAATGACCCGGC	AGCGCAGAATTGGTTGAAGA	50.0	45.0	58.2	58.1	839-849 bp	1.0	78.1
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	807-817 bp	1.0	77.9
psaA-ycf3_p5	TTCTTGAGAAATGACCCGGC	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.2	57.8	826-836 bp	1.0	77.6