Hotspot

petN-psbM

Genome · 48609-49766 · 1158 bp

Back to Dystaenia

Pi 0.00524

MarkerSeek score 62.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: Genome
length_bp: 1158
variable_sites: 6
pi: 0.00524
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATCC	50.0	50.0	58.2	57.9	1332-1346 bp	1.0	79.1
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATCC	50.0	50.0	57.6	57.9	1271-1285 bp	1.0	77.5
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	GCTACTGCACTGTTCATCCT	50.0	50.0	58.2	57.6	1331-1345 bp	1.0	76.7
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	GCTACTGCACTGTTCATCCT	50.0	50.0	57.6	57.6	1270-1284 bp	1.0	75.2
petN-psbM_p5	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATCC	50.0	50.0	57.1	57.9	1333-1347 bp	1.0	73.1