Hotspot

ycf1

SSC · 122478-128042 · 5565 bp

Back to Dysphania

Pi 0.0391

MarkerSeek score 64.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5565
variable_sites: 321
pi: 0.0391
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATTCCACTTCGTCTGCTTCC	AAACCCCAGAAACGAACGAA	50.0	45.0	57.9	58.0	1344-1347 bp	1.0	84.2
ycf1_p2	ATTCCACTTCGTCTGCTTCC	AACCCCAGAAACGAACGAAA	50.0	45.0	57.9	58.0	1343-1346 bp	1.0	84.2
ycf1_p3	CCCTTTCCTTGAGCGAAGTT	AAACCCCAGAAACGAACGAA	50.0	45.0	58.1	58.0	1250-1253 bp	1.0	83.8
ycf1_p4	CCCTTTCCTTGAGCGAAGTT	AACCCCAGAAACGAACGAAA	50.0	45.0	58.1	58.0	1249-1252 bp	1.0	83.8
ycf1_p5	TTCCCTTTCCTTGAGCGAAG	AACCCCAGAAACGAACGAAA	50.0	45.0	57.8	58.0	1251-1254 bp	1.0	83.1