Hotspot

rpl22

LSC · 82621-83208 · 588 bp

Back to Dysphania

Pi 0.0684

MarkerSeek score 79.80

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 588
variable_sites: 58
pi: 0.0684
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	ATCGATACGGCCTGCAATTT	TGGGTAGAGCCGGATCTAAG	45.0	55.0	58.0	58.0	1744-1755 bp	1.0	85.0
rpl22_p2	ACTAGGGTGCCCATAAGGTT	AAGCTTAACACAAAGGCGGA	50.0	45.0	58.0	58.0	1268-1279 bp	0.666667	71.0
rpl22_p3	ACTAGGGTGCCCATAAGGTT	AGCTTAACACAAAGGCGGAA	50.0	45.0	58.0	58.0	1267-1278 bp	0.666667	71.0
rpl22_p4	ACTAGGGTGCCCATAAGGTT	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	1612-1623 bp	0.666667	70.2
rpl22_p5	GCAAGAATACTAGGGTGCCC	TGGGTAGAGCCGGATCTAAG	55.0	55.0	58.0	58.0	1620-1631 bp	0.666667	70.1