Hotspot

rrn23-rrn4.5

IRb · 105174-105278 · 105 bp

Back to Dysphania

Pi 0.216

MarkerSeek score 85.30

Primer available yes

Label rrn23-rrn4.5

Feature Metadata

label_name: rrn23-rrn4.5
feature_type: igs
region: IRb
length_bp: 105
variable_sites: 32
pi: 0.216
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23-rrn4.5_p1	AAGTAGTAAGCCCACCCCAA	CTGTGGGTCTGTTAGGATGC	50.0	55.0	58.2	58.0	236-283 bp	1.0	89.8
rrn23-rrn4.5_p2	AAGTAGTAAGCCCACCCCAA	TCACTGCACTTCCACTTGAC	50.0	50.0	58.2	58.0	202-249 bp	1.0	89.6
rrn23-rrn4.5_p3	AAGTAGTAAGCCCACCCCAA	CTGTTAGGATGCCTCAGCTG	50.0	55.0	58.2	58.0	228-275 bp	1.0	89.6
rrn23-rrn4.5_p4	AAGTAGTAAGCCCACCCCAA	GTCTGTTAGGATGCCTCAGC	50.0	55.0	58.2	58.1	230-277 bp	1.0	89.6
rrn23-rrn4.5_p5	TAGTAAGCCCACCCCAAGAT	CTGTGGGTCTGTTAGGATGC	50.0	55.0	57.7	58.0	233-280 bp	1.0	89.5