Hotspot

trnP-UGG-psaJ

LSC · 65783-66148 · 366 bp

Back to Dysphania

Pi 0.0474

MarkerSeek score 78.50

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 366
variable_sites: 26
pi: 0.0474
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	TGGTGCCACGGAAAGATATG	50.0	50.0	58.1	58.0	457-464 bp	1.0	85.3
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	TGGTGCCACGGAAAGATATG	50.0	50.0	58.1	58.0	456-463 bp	1.0	85.3
trnP-UGG-psaJ_p3	ACCCGTGACATTTTGTACCC	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	456-463 bp	1.0	85.3
trnP-UGG-psaJ_p4	CCCGTGACATTTTGTACCCA	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	455-462 bp	1.0	85.3
trnP-UGG-psaJ_p5	ACAGGATTTGAACCCGTGAC	TGGTGCCACGGAAAGATATG	50.0	50.0	58.1	58.0	468-475 bp	1.0	85.1