Hotspot

ndhC-trnV-UAC

LSC · 49901-50726 · 826 bp

Back to Dysphania

Pi 0.0541

MarkerSeek score 85.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 826
variable_sites: 63
pi: 0.0541
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGCCCAGAAAATATCATATTCA	GTTCGAGTCCGTATAGCCCT	31.8	55.0	53.3	58.7	842-886 bp	1.0	49.6
ndhC-trnV-UAC_p2	TGCCCAGAAAATATCATATTCA	AGAAGGTCTACGGTTCGAGT	31.8	50.0	53.3	57.8	854-898 bp	1.0	49.4
ndhC-trnV-UAC_p3	TGCCCAGAAAATATCATATTCA	GAAGGTCTACGGTTCGAGTC	31.8	55.0	53.3	57.5	853-897 bp	1.0	49.4
ndhC-trnV-UAC_p4	ATGCCCAGAAAATATCATATTCA	AGAAGGTCTACGGTTCGAGT	30.4	50.0	53.9	57.8	855-899 bp	1.0	49.4
ndhC-trnV-UAC_p5	TGCCCAGAAAATATCATATTCAT	AGAAGGTCTACGGTTCGAGT	30.4	50.0	53.9	57.8	854-898 bp	1.0	49.4