Hotspot

trnT-UGU-trnL-UAA

LSC · 45742-46568 · 827 bp

Back to Dysphania

Pi 0.0422
MarkerSeek score 81.80
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
827
variable_sites
49
pi
0.0422
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG AGCCAATTAAGTCCGTAGCG 55.0 50.0 57.9 58.1 870-901 bp 1.0 86.9
trnT-UGU-trnL-UAA_p2 GACTCGAACCGATGACCATC AGCCAATTAAGTCCGTAGCG 55.0 50.0 58.2 58.1 901-932 bp 1.0 86.5
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 858-889 bp 1.0 85.5
trnT-UGU-trnL-UAA_p4 GACTCGAACCGATGACCATC CCGTAGCGTCTACCGATTTC 55.0 55.0 58.2 58.2 889-920 bp 1.0 85.1
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG GCCAATTAAGTCCGTAGCGT 55.0 50.0 57.9 58.4 869-900 bp 1.0 84.6