Hotspot

petN-psbM

LSC · 27578-28707 · 1130 bp

Back to Dysphania

Pi 0.0572
MarkerSeek score 78.60
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1130
variable_sites
95
pi
0.0572
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TTTTGTATCGTTTTGGCGGC CACTCGCGTCTCTGTTACAA 45.0 50.0 57.9 58.0 2422-2500 bp 1.0 86.1
petN-psbM_p2 TTTTGTATCGTTTTGGCGGC CTCGCGTCTCTGTTACAACA 45.0 50.0 57.9 58.0 2420-2498 bp 1.0 86.1
petN-psbM_p3 AACAAAAGACTCGAACCCCC CACTCGCGTCTCTGTTACAA 50.0 50.0 58.0 58.0 2327-2405 bp 0.666667 70.9
petN-psbM_p4 AACAAAAGACTCGAACCCCC CTCGCGTCTCTGTTACAACA 50.0 50.0 58.0 58.0 2325-2403 bp 0.666667 70.9
petN-psbM_p5 AACAAAAGACTCGAACCCCC ACTCGCGTCTCTGTTACAAC 50.0 50.0 58.0 58.0 2326-2404 bp 0.666667 70.9