Hotspot

psbK-psbI

LSC · 6960-7390 · 431 bp

Back to Dysphania

Pi 0.0504

MarkerSeek score 82.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 431
variable_sites: 31
pi: 0.0504
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	AGGAATCCGAAAATGAAGAGAGA	50.0	39.1	57.9	57.3	517-532 bp	1.0	58.4
psbK-psbI_p2	CTTTTGTTTGGCAAGCTGCT	AGGAATCCGAAAATGAAGAGAGA	45.0	39.1	58.1	57.3	522-537 bp	1.0	58.3
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	AGGAATCCGAAAATGAAGAGAGA	45.0	39.1	58.3	57.3	514-529 bp	1.0	56.4
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	AGGAATCCGAAAATGAAGAGAGA	45.0	39.1	58.3	57.3	515-530 bp	1.0	56.4
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	AGGAATCCGAAAATGAAGAGAGA	45.0	39.1	58.3	57.3	516-531 bp	1.0	56.3