Hotspot

rps16-trnQ-UUG

LSC · 5786-6387 · 602 bp

Back to Dysphania

Pi 0.0598
MarkerSeek score 81.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
602
variable_sites
54
pi
0.0598
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 675-679 bp 1.0 84.7
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA TAAGGCATCGGGTTTTGGTC 45.0 50.0 58.1 58.2 705-709 bp 1.0 83.6
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GTAAGGCATCGGGTTTTGGT 45.0 50.0 58.1 58.5 706-710 bp 1.0 81.3
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GTGGTAAGGCATCGGGTTTT 45.0 50.0 58.1 58.5 709-713 bp 1.0 81.3
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TGGTAAGGCATCGGGTTTTG 45.0 50.0 58.1 58.5 708-712 bp 1.0 81.3