Hotspot

rrn16

IRa · 139733-141223 · 1491 bp

Back to Dysosma

Pi 0.0427

MarkerSeek score 64.60

Primer available yes

Label rrn16

Feature Metadata

label_name: rrn16
feature_type: rRNA
region: IRa
length_bp: 1491
variable_sites: 127
pi: 0.0427
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16_p1	GGCTCCAAGTTGTTCAGGAA	TGAATCGCCCCATCTATCCT	50.0	50.0	58.0	58.0	2390-2393 bp	1.0	86.2
rrn16_p2	GGCTCCAAGTTGTTCAGGAA	CAGACGTAGCTCGCTTCTTT	50.0	50.0	58.0	58.0	1962 bp	0.75	69.8
rrn16_p3	GAGCAAACTCCAGGCGAATA	CAGACGTAGCTCGCTTCTTT	50.0	50.0	58.0	58.0	1682 bp	0.75	69.7
rrn16_p4	GCAAGAAGAAGGCCGTATCA	CAGACGTAGCTCGCTTCTTT	50.0	50.0	58.0	58.0	2246 bp	0.75	69.7
rrn16_p5	GAGCAAACTCCAGGCGAATA	TGAATCGCCCCATCTATCCT	50.0	50.0	58.0	58.0	2113 bp	0.75	69.6
rrn16_p1	TGAATCGCCCCATCTATCCT	GAGCAAACTCCAGGCGAATA	50.0	50.0	58.0	58.0	2110-2113 bp	1.0	86.2
rrn16_p2	CAGACGTAGCTCGCTTCTTT	GGCTCCAAGTTGTTCAGGAA	50.0	50.0	58.0	58.0	1962 bp	0.75	69.8
rrn16_p3	CAGACGTAGCTCGCTTCTTT	GAGCAAACTCCAGGCGAATA	50.0	50.0	58.0	58.0	1682 bp	0.75	69.7
rrn16_p4	CAGACGTAGCTCGCTTCTTT	GCAAGAAGAAGGCCGTATCA	50.0	50.0	58.0	58.0	2246 bp	0.75	69.7
rrn16_p5	TGAATCGCCCCATCTATCCT	GGCTCCAAGTTGTTCAGGAA	50.0	50.0	58.0	58.0	2393 bp	0.75	69.6