Hotspot

ndhF-rpl32

SSC · 115249-116117 · 869 bp

Back to Dysosma

Pi 0.014

MarkerSeek score 65.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 869
variable_sites: 22
pi: 0.014
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCGGAAGGAAGGGTATGATC	GTACGTTTCTTTGGAACCGC	55.0	50.0	57.8	57.7	928-941 bp	1.0	79.8
ndhF-rpl32_p2	AGCGGAAGGAAGGGTATGAT	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.2	57.7	929-942 bp	1.0	79.6
ndhF-rpl32_p3	GCGGAAGGAAGGGTATGATC	GAAAGCGCTTTTACTGCCAC	55.0	50.0	57.8	58.3	1003-1016 bp	1.0	79.4
ndhF-rpl32_p4	AGTCCCACTCCGATTGCTAT	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.2	57.7	959-972 bp	1.0	79.4
ndhF-rpl32_p5	AGCGGAAGGAAGGGTATGAT	GAAAGCGCTTTTACTGCCAC	50.0	50.0	58.2	58.3	1004-1017 bp	1.0	79.3