Hotspot

ndhC-trnV-UAC

LSC · 52635-54279 · 1645 bp

Back to Dysosma

Pi 0.0229

MarkerSeek score 64.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1645
variable_sites: 56
pi: 0.0229
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	36.4	50.0	56.1	57.8	1591-1701 bp	1.0	54.1
ndhC-trnV-UAC_p2	TCATATTCGTGAAGCAGAAACA	AGTGCATGTTGGGTCTTTGA	36.4	45.0	56.1	57.6	1658-1768 bp	1.0	52.6
ndhC-trnV-UAC_p3	TCATATTCGTGAAGCAGAAACA	GAAGGTCTACGGTTCGAGTC	36.4	55.0	56.1	57.5	1590-1700 bp	1.0	51.3
ndhC-trnV-UAC_p4	TCATATTCGTGAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	36.4	55.0	56.1	58.7	1579-1689 bp	1.0	50.2
ndhC-trnV-UAC_p5	CATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	38.1	50.0	54.6	57.8	1590-1700 bp	1.0	50.0