Hotspot

trnT-UGU-trnL-UAA

LSC · 48386-49225 · 840 bp

Back to Dysosma

Pi 0.0135

MarkerSeek score 67.50

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 840
variable_sites: 20
pi: 0.0135
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	55.0	50.0	57.9	57.8	882-913 bp	1.0	82.9
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	870-901 bp	1.0	82.8
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CAATCAAGTCCGTAGCGTCT	55.0	50.0	57.9	57.7	879-910 bp	1.0	82.5
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	ATCCAATCAAGTCCGTAGCG	50.0	50.0	57.8	57.8	914-945 bp	1.0	82.0
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	902-933 bp	1.0	81.8