Hotspot

trnS-GCU-trnG-GCC

LSC · 8124-8801 · 678 bp

Back to Dysosma

Pi 0.0161

MarkerSeek score 66.80

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 678
variable_sites: 21
pi: 0.0161
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	AACCGAAAGACCCCTTAACT	45.0	45.0	58.0	56.4	788-800 bp	1.0	69.5
trnS-GCU-trnG-GCC_p2	AAGGAACGGAAAGAGAGGGA	AACCGAAAGACCCCTTAACT	50.0	45.0	58.0	56.4	842-854 bp	1.0	69.4
trnS-GCU-trnG-GCC_p3	ACGGAAAGAGAGGGATTCGA	AACCGAAAGACCCCTTAACT	50.0	45.0	58.2	56.4	837-849 bp	1.0	68.5
trnS-GCU-trnG-GCC_p4	AGGAACGGAAAGAGAGGGAT	AACCGAAAGACCCCTTAACT	50.0	45.0	57.7	56.4	841-853 bp	1.0	67.6
trnS-GCU-trnG-GCC_p5	AAGGAACGGAAAGAGAGGGA	AAACCGAAAGACCCCTTAACT	50.0	42.9	58.0	57.1	843-855 bp	1.0	67.6