Hotspot

ycf1

SSC · 127589-133006 · 5418 bp

Pi 0.0151

MarkerSeek score 60.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5418
variable_sites: 189
pi: 0.0151
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGTCTTTGAGTACGCATGGA	TTTATTGAGCGGACCATCCC	50.0	50.0	58.0	57.7	666 bp	0.6	62.8
ycf1_p2	TGGGGGAAATTCTGTTTCCG	TTTATTGAGCGGACCATCCC	50.0	50.0	58.1	57.7	1138 bp	0.6	62.2
ycf1_p3	ATGCGCATTTGCTTGAAACA	TTTATTGAGCGGACCATCCC	40.0	50.0	57.8	57.7	706 bp	0.6	61.5
ycf1_p4	GGGGGAAATTCTGTTTCCGA	TTTATTGAGCGGACCATCCC	50.0	50.0	57.8	57.7	1137 bp	0.6	61.2
ycf1_p5	CAAATCCTCAGACCACTCGG	TTTATTGAGCGGACCATCCC	55.0	50.0	58.0	57.7	1063 bp	0.4	54.1