Hotspot

rps19

IRb · 87251-87529 · 279 bp

Back to Dypsis

Pi 0.00143
MarkerSeek score 24.60
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
IRb
length_bp
279
variable_sites
1
pi
0.00143
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
24.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 GCGAACCAAAAGGAACGATG GTTATCCTGCGCTTGGAAGA 50.0 50.0 58.0 58.0 1262-1283 bp 1.0 82.3
rps19_p2 TGCTGCTGCAGAATAAACCA GGGTAGAGCCGGATCTAAGT 45.0 55.0 58.1 58.0 1084-1105 bp 1.0 82.3
rps19_p3 GCGAACCAAAAGGAACGATG GGGTAGAGCCGGATCTAAGT 50.0 55.0 58.0 58.0 1399-1420 bp 1.0 82.2
rps19_p4 GCGAACCAAAAGGAACGATG TGGGTAGAGCCGGATCTAAG 50.0 55.0 58.0 58.0 1400-1421 bp 1.0 82.2
rps19_p5 GCGAACCAAAAGGAACGATG GAGGGTAAATCGGCCACATT 50.0 50.0 58.0 57.9 1512-1533 bp 1.0 81.6