Hotspot

accD

LSC · 59998-61476 · 1479 bp

Pi 0.0113

MarkerSeek score 59.00

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1479
variable_sites: 35
pi: 0.0113
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATAGGGGGCAAGAAGACTCT	AGAAAACACCATCCTTTTTCGT	50.0	36.4	57.4	56.7	1557-1584 bp	1.0	52.4
accD_p2	ATAGGGGGCAAGAAGACTCT	TGTCATCAAAGAAAACACCATCC	50.0	39.1	57.4	57.5	1566-1593 bp	1.0	50.9
accD_p3	ATAGGGGGCAAGAAGACTCT	AAGAAAACACCATCCTTTTTCGT	50.0	34.8	57.4	57.4	1558-1585 bp	1.0	49.8
accD_p4	ATAGGGGGCAAGAAGACTCT	AGAAAACACCATCCTTTTTCGTT	50.0	34.8	57.4	57.4	1557-1584 bp	1.0	49.8
accD_p5	ATAGGGGGCAAGAAGACTCT	GTCATCAAAGAAAACACCATCCT	50.0	39.1	57.4	57.3	1565-1592 bp	1.0	48.8