Hotspot

ycf2-trnL-CAA

IRb · 96782-97800 · 1019 bp

Back to Dypsis

Pi 0.00236
MarkerSeek score 61.10
Primer available yes
Label ycf2-trnL-CAA

Feature Metadata

label_name
ycf2-trnL-CAA
feature_type
igs
region
IRb
length_bp
1019
variable_sites
6
pi
0.00236
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnL-CAA_p1 AAAGAGATGGCTTTTCCCGG AAATGGTAGACACGCGAGAC 50.0 50.0 58.2 58.0 1161-1170 bp 1.0 79.4
ycf2-trnL-CAA_p2 AAAGAGATGGCTTTTCCCGG TGAAATGGTAGACACGCGAG 50.0 50.0 58.2 58.0 1163-1172 bp 1.0 79.4
ycf2-trnL-CAA_p3 ATGGCTTTTCCCGGATGAAA AAATGGTAGACACGCGAGAC 45.0 50.0 57.8 58.0 1155-1164 bp 1.0 78.9
ycf2-trnL-CAA_p4 TGGCTTTTCCCGGATGAAAT AAATGGTAGACACGCGAGAC 45.0 50.0 57.8 58.0 1154-1163 bp 1.0 78.9
ycf2-trnL-CAA_p5 TGGCTTTTCCCGGATGAAAT TGAAATGGTAGACACGCGAG 45.0 50.0 57.8 58.0 1156-1165 bp 1.0 78.8