Hotspot

ndhC-trnV-UAC

LSC · 52103-53993 · 1891 bp

Back to Dypsis

Pi 0.00461
MarkerSeek score 64.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1891
variable_sites
21
pi
0.00461
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCTTCACTAACCGGTGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.1 57.8 1995-2041 bp 1.0 80.9
ndhC-trnV-UAC_p2 TAACCGGTGCTAAAACTCCG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1987-2033 bp 1.0 80.8
ndhC-trnV-UAC_p3 TCCTTCACTAACCGGTGCTA AGCCCAATGCAACAAAATGC 50.0 45.0 58.1 58.5 2077-2123 bp 1.0 78.5
ndhC-trnV-UAC_p4 TAACCGGTGCTAAAACTCCG AGCCCAATGCAACAAAATGC 50.0 45.0 57.9 58.5 2069-2115 bp 1.0 78.5
ndhC-trnV-UAC_p5 TCCTTCACTAACCGGTGCTA GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.1 57.5 1994-2040 bp 1.0 78.1