Hotspot

trnS-GCU-trnG-UCC

LSC · 8391-9731 · 1341 bp

Back to Dypsis

Pi 0.0132
MarkerSeek score 62.60
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1341
variable_sites
40
pi
0.0132
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA ACGAATCACACTTTTACCACT 45.0 38.1 58.0 55.5 1415-1583 bp 1.0 56.1
trnS-GCU-trnG-UCC_p2 ATTAGCAATCCGCCGCTTTA AGAACGAATCACACTTTTACCAC 45.0 39.1 58.0 57.3 1418-1586 bp 1.0 55.1
trnS-GCU-trnG-UCC_p3 ATTAGCAATCCGCCGCTTTA GAACGAATCACACTTTTACCACT 45.0 39.1 58.0 57.3 1417-1585 bp 1.0 55.1
trnS-GCU-trnG-UCC_p4 ATTAGCAATCCGCCGCTTTA AACGAATCACACTTTTACCACT 45.0 36.4 58.0 56.2 1416-1584 bp 1.0 54.2
trnS-GCU-trnG-UCC_p5 GCTTTAGTCCACTCAGCCAT ACGAATCACACTTTTACCACT 50.0 38.1 57.6 55.5 1401-1569 bp 1.0 53.2